Tracy: basecalling, alignment, assembly and deconvolution of sanger chromatogram trace files
نویسندگان
چکیده
منابع مشابه
Trace modelling for abduction basecalling
DNA sequencing using the fluoresence based Sanger method comprises interpretation of a sequence of signal peaks of varying size whose colour indicates the presence of a base. We have established that the ability to predict the variations effectively makes available novel error correction information which will improve sequencing efficacy. Our experiments so far have used basic models of the San...
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DNA sequence basecalling is commonly regarded as a solved problem, despite significant error rates being reflected in inaccuracies in databases and genome annotations. These errors commonly arise from an inability to sequence through peak height variations in DNA sequencing traces from the Sanger sequencing method. Recent efforts toward improving basecalling accuracy have taken the form of more...
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In DNA (deoxyribonucleic acid) sequencing, there are four possible chemical base types: adenine (A), cytosine (C), guanine (G), thymine (T), which contain genetic information. The four base types are identified by examining four DNA electrophoresis time series. This procedure is called “basecalling”. However, in practice, there are many other undesired signal features that prevent the accurate ...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2020
ISSN: 1471-2164
DOI: 10.1186/s12864-020-6635-8